Tecan Freedom EVO-2 200 Liquid Handler w/ LiHa Span8, MCA-96, ROMA Gripper in Walpole, MA, USA

- WARRANTY

Optional Services

- Optional on-site setup and training are available for customers within 2 hours of Boston, MA
- Optional remote installation support and training are available for non-local customers

Service Highlights

- Calibration beads test results match expected values
- Laser power output confirmed stable over time
- Instrument stress testing performed

Configuration Highlights

- Includes 3 Laser V/B/R, 96-well plate sampler
- Includes a complementary subscription to De Novo FCS Express 7 Analysis software
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Here is what you will receive:

- Cytek Aurora Flow Cytometer (3 lasers)
- APL Automated Plate Loader N7-00020
- Desktop PC running SpectroFlo software version 3.3.0
- (2) 4-L Fluid Containers for sheath and waste solution
- Complimentary De Novo FCS Express analysis software (1-Year Subscription) RUO version
- Communication and power cables

When you purchase a flow cytometer from Boston Industries, you also receive a complimentary 1-year subscription to De Novo FCS Express 7 Analysis software, enhancing your research capabilities with these key benefits:

- User-Friendly Interface: Navigate complex data analysis easily using FCS Express's intuitive design.
- Advanced Analysis Tools: Utilize sophisticated tools for in-depth data exploration and interpretation.
- Comprehensive Reporting: Generate detailed, customizable reports that are publication-ready.
- High Compatibility: Supports multiple file formats, including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter files, Accuri C6 files, SPADE, Cytobank files, and TXT files.
- Enhanced Data Visualization: Create high-quality graphs and plots to visualize your data effectively
- With FCS Express 7, you will have the latest Research Use analysis software.

Cytek Aurora Flow Cytometer Configuration:

- Optics: (3)Lasers/(38)Fluorescence Channels/(41)Detector
- Laser/Channel Count: 16V-14B-8R
- Violet (405 nm) detector module: 16 channels, unevenly spaced bandwidth from 420-829 nm
- Blue (488 nm) detector module: 14 channels, unevenly spaced bandwidth from 498-829 nm
- Red (640 nm) detector module: 8 channels, unevenly spaced bandwidth from 652-829 nm
- Forward scatter detector and filter: high-performance semiconductor detector with 488 nm bandpass filter
- Side scatter detectors and filters: (2) high-performance semiconductor detectors with 405 nm and 488 nm bandpass filters
- General operation: vacuum-driven fluidics with the following fluidics modes: Long Clean, SIT Flush, Purge Filter, Clean Flow Cell, Fluidics Shutdown
- Sample input: manual (12x75 mm polystyrene and polypropylene tubes), plate loader (96-well microtiter plate)
- Sample flow rates:
- Tube mode: low: 15 uL/min, medium: 30 uL/min, high: 60 uL/min
- Plate mode: low: 15 uL/min, medium: 30 uL/min, high: 100 uL/min
- Data acquisition rate: up to 35,000 events/s

Customization/Options: N/A

Testing performed by our in-house service team:

The Cytek Aurora Flow Cytometer passed all tests and was found to be fully functional:

- We powered on the Cytek Aurora Flow Cytometer and verified startup functionality.
- We successfully established a connection with the control software.
- We performed the SIT calibration to ensure proper system alignment.
- We carried out multiple flow cell cleaning cycles for optimal fluidics performance.
- We confirmed full operational functionality of the sample loader.
- We ran quality control using SpectraFlo Beads, with all results passing within specification.

Instrument Overview:

- The Cytek Aurora Flow Cytometry System offers full-spectrum detection across fluorescence channels.
- Features a 3-laser configuration enabling high-parameter analysis with minimal spectral overlap.
- Equipped with highly sensitive detectors for precise signal resolution and data quality.
- Includes an automated plate loader for efficient high-throughput sample acquisition.
- The Cytek Aurora is optimized for complex, multiparameter experiments in a compact footprint.
- Aurora’s advanced optics support detection of rare populations and dim signals with clarity.

Instrument Application:

- Deep immunophenotyping of complex cell populations
- High-throughput screening of PBMCs or tumor samples
- Detection of rare immune subsets in whole blood
- Multiparameter analysis of T-cell activation markers
- Fluorescent protein expression profiling in transfected cells

The key features:

- 3-laser system for flexible excitation
- 38-channel full-spectrum detection
- 41 detectors for high resolution
- Dual color side scatter detection Violet/Blue (405/488)
- Automated plate loader included
- Compact design saves bench space
- Ideal for high-parameter analysis

De Novo FCS Express Software Features

- Data Import and Handling: Easily imports data from various flow cytometry instruments, supporting a wide range of file formats.
- Intuitive Interface: User-friendly interface with customizable dashboards for streamlined workflow management.
- Advanced Data Analysis: Provides comprehensive tools for complex data analysis, including statistics, gating, and kinetic analysis.
- Visualization Tools: Offers a variety of plotting options, such as histograms, scatter plots, and heat maps, for effective data visualization.
- Automated Reporting: Facilitates the creation of detailed reports with integrated data and graphics, automating repetitive tasks.
- Real-Time Data Updates: Supports real-time data analysis and visualization, enabling immediate insights and decision-making.
- Collaboration Features: Allows sharing of data and analysis results with team members through secure cloud-based platforms.
- Integration Capabilities: Seamlessly integrates with other laboratory software and databases for efficient data management and workflow integration.

Benefits of the Complimentary DeNovo FCS Express Software

- Enhanced Data Management: Streamlines data import, handling, and analysis, improving overall data management efficiency.
- User-Friendly Design: Intuitive interface design ensures ease of use, reducing the learning curve and increasing productivity.
- Comprehensive Analysis Tools: Advanced analysis tools allow for in-depth examination of complex data sets, leading to more accurate results.
- Flexible Visualization Options: Wide range of plotting options helps in effectively visualizing data, facilitating better understanding and presentation.
- Time-Saving Automation: Automated reporting and analysis features save time and reduce manual effort, enhancing workflow efficiency.
- Immediate Insights: Real-time data updates enable quick insights and decision-making, crucial for time-sensitive experiments.
- Collaboration and Sharing: Secure cloud-based collaboration features promote data sharing and teamwork, enhancing research collaboration.
- Seamless Integration: Integration capabilities with other software and databases ensure smooth workflow and data management.

Additional technical info:

- Analysis Software: FCS Express Analysis Software
- Brochure: Cytek Aurora Flow Cytometer Brochure
- Manual: Cytek Aurora Flow Cytometer Manual

Note: Subscription is for the Research Use Only (RUO) version.

CRITICAL TECHNICAL STEPS WE TAKE TO ENSURE INSTRUMENT PERFORMANCE & CUSTOMER SATISFACTION

They want to ensure the cytometer undergoes full fluidics system decontamination, flushing, and inspection to protect the valves and flow sensors from damage.

They want to ensure the cytometer has no residual sheath salt buildup in the flow cell, which could skew scatter data and, and if improperly cleaned, can damage the flow cell.

They want to confirm that all detectors, including forward scatter (FSC) and side scatter (SSC) detectors, can be aligned and calibrated for particle sizing and granularity resolution.

They want assurance that baseline performance has been verified using standardized particles, with data or log files available for review.

They want to confirm that electronic noise and EMI levels at the detector/amplifier stage remain within tolerance and will not distort signal fidelity.

They want to ensure that no fiber optic cables are missing, misrouted, or defective, which can lead to diminished fluorophore excitation or signal dropout.

They want to confirm that no power degradation or misalignment in laser paths is detected through precise mW-level measurement at the final optics output.

They want to ensure that optical filters and dichroic mirrors have been inspected for delamination, spectral shift, or contamination that could compromise signal specificity.

They want to ensure the sample flow rate is linear and stable across a full range of flow settings, verified with volumetric calibration and pressure profiling.

They want to ensure the system passes automated QC and performance tracking with reproducible CVs for bead standards across multiple channels.

They want to ensure the fluidics pump assembly does not exhibit pulsation, drift, or backpressure due to check valve wear or micro-leaks.

They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

Our Advantage

- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee

- Warranty
- An extended warranty may be available
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?

A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?

A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?

A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?

A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?

A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?

A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?

A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?

A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?

A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.

Q: Do you ship Internationally?

A: Yes! We have shipped to multiple continents and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?

A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.